Axenic cultures and defined media

  • 272 Pages
  • 2.46 MB
  • English
The Academy , New York
Biology -- Research., Cultures (Biology), Germfree
Other titlesDefined media
Statementconsulting editor: J.D. Tiner. Authors: J.D. Tiner [and others] Editor-in-chief: Edward M. Weyer.
SeriesAnnals -- v. 139, art. 1, Annals of the New York Academy of Sciences -- v. 139
ContributionsTiner, J. D., Weyer, Edward Moffat, 1904-1998
LC ClassificationsQH324 N4
The Physical Object
ID Numbers
Open LibraryOL16749909M

Axenic Cultures and Defined Media Paperback – January 1, by Editor Weyer, Edward M. (Author) See all formats and editions Hide other formats and editions.

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From a practical viewpoint, because the contaminating organisms usually encountered are microorganisms, axenic cultures, whether of invertebrates (for example, nematodes) or vertebrates (for example, rodents), are often referred to as germ-free.

Indeed, the terms axenic and germ-free are occasionally used interchangeably. Abstract. The defined and semi-defined liquid and agar-based media reported for the culture of Physarum polycephalum in the early s (Daniel and Baldwin, ) were successful in supporting the growth of a wide range of plasmodial strains but apparently failed to support the growth of myxamoebae.

By the s, several liquid synthetic media had been devised for the culture of amoebae of Cited by: 7. Cite this entry as: Mehlhorn H. () Axenic Cultures. In: Mehlhorn H. (eds) Encyclopedia of Parasitology. Springer, Berlin, Axenic to Mixed Cultures: Technological Advances Accelerating a Paradigm Shift in Microbiology Corrado Nai1,2,@ and Axenic cultures and defined media book Meyer1,* Since the onset of microbiology in the late 19th century, scientists have been growing microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of the microbial world.

Only a. axenic culture: a pure culture of microorganisms, i.e., one free from contaminating microorganisms or, in the case of parasites, without the presence of the host. In plant tissue culture, culture contamination is the major problem encountered for the establishment of viable culture using field-grown plant materials.

The main objective of this study is to develop effective protocol for the axenic (contamination-free) culture establishment of Ramin in Size: KB.

Are there any methods available (media, growth conditions) for growing axenic cultures of Russula fungi, apart from the paper sandwich method (Chilvers et al ) and the floating culture.

Author(s) Understanding Media and Culture: An Introduction to Mass Communication is adapted from a work produced by a publisher who Communication, Media, and Culture - Table of ContentsThis is the Axenic cultures and defined media book of contents for the book Mass Communication, Media, and Culture (v.

Agnotoxenic and heteroxenic animals deriving from axenic individuals are usually defined as ‘specific pathogen free’ or ‘specified pathogen free’ (SPF). Originally, ‘specific’ refers to the absence of ‘species-specific’ pathogenic agents, whereas ‘specified’ implies a predefined and specified exclusion list.

() used a defined axenic culture; however, the nematode growth in the defined axenic medium was poor and yield was far less than monoxenic cultures with symbiotic bacteria on artificial diets. On the other hand, nematode growth and propagation are better in axenic culture using rat liver or.

Define Axenic culture. Axenic culture synonyms, Axenic culture pronunciation, Axenic culture translation, English dictionary definition of Axenic culture.

Description Axenic cultures and defined media EPUB

adj. Not contaminated by or associated with any other living organisms. Usually used in reference to pure cultures of. Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology.

It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or multicellular organisms.

Samuel Goldenberg, Andrea Rodrigues Ávila, in Advances in Parasitology, Trypanosoma cruzi Differentiation. Most studies concerning the requirements and factors affecting T.

cruzi differentiation focused on the metacyclogenesis process due to the availability of axenic culture conditions, as described above. There are several factors involved in triggering T.


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Cultures of Naegleria and Acanthamoeba can be maintained with bacteria as a food source (xenic cultures) or, by addition of antibiotics to destroy bacteria, can be established in cell-free (axenic) cultures (Fig.

2A and B). Appropriate media for axenic cultivation are described above in the section on axenic by: A range of media have been proposed for axenic S. indica propagation, but these media are complex and can be somewhat laborsome to prepare with particular technical requirements [14, 15] or are.

Navigating today’s hyper-fast media landscape. The #1 introduction to mass communication text, Media & Culture: Mass Communication in a Digital Age, is at the forefront of the ever-changing world of this dynamic course, addressing the most current issues of our time—including the proliferation of fake news, the #metoo movement, the use and abuse of social media platforms, consumer privacy.

Ethics and Media Culture straddles the practical and ethical issues of contention encountered by journalists. The book's various contributors cover a diversity of issues and viewpoints, attempting to broaden out the debates particularly in relation to Journalism Studies, Cultural Studies, Sociology of Culture and Communications, Philosophy and by:   A pure (or axenic) culture is a population of cells or multicellular organisms growing in the absence of other species or types.

A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. Culture Collection of Algae Vers.

Soil Water Media These media have great advantages for many purposes as long as axenic culture is not required, e.g. for the cultivation of species of which nutrient requirements are not known, or to obtain morphologically normal growth.

This book is intended for final year undergraduates and postgraduates in cultural and media studies, as well as postgraduate and academic researchers. Courses on culture and the media within sociology, environmental studies, human geography and by: Pl P General Mycology medium for growing cultures of Agaricus, Pleurotus, Lentinus, Stropharia, Flammulina, and some of the Psilocybe species.

Summary of media and common use Water Agar (WA)--use for isolating fungi from surface-sterilized substrates. Antibiotic Agar (AA)--use for isolating fungi from substrates not readily surface- sterilized, or to clean up a culture contaminated with.

Favored RLE for use in promoting growth and development of Neoaplectana glaseri in axenic cultures include those made from the livers of rabbits sacrificed late in pregnancy or early postpartum, and used after subsequent refrigerated storage periods of 15–90 by: 5.

A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as a research tool in molecular biology.

Microbial cultures are used to determine the type of organism, its abundance in the sample. Define or explain: i) Aseptic Technique ii) Axenic Culture iii) Complex or Nutrient media iv) Anaerobic v) Defined/Synthetic media Get more help from Chegg Get help now from expert Biology tutors. Comparison of axenic and monoxenic media for isolation of Acanthamoeba.

Penland RL(1), Wilhelmus KR. Author information: (1)Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Baylor College of Medicine, Houston, Texasby: a pure culture is the one that originate from a single cell and has same specie of an organism while axenic culture can originate from more than one cell.

In addition, to get a better understanding of a culture, we often need to look beyond the media. We can read books about different cultures and visit different cultural museums.

To summarize, culture is the beliefs, customs, arts, language, and more of a particular group of people. "Axenic Culture" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity.

Growth from uredospores seeded in axenic culture is described for several races of Puccinia graminis Pers. f. sp.

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tritici (Erikss. and Henn.) and race 3 of Melampsora lini (Ehrenb.) Lév. on complex media containing peptone, yeast extract, and bovine serum albumin (BSA); and for an Australian isolate of Puccinia graminis, race ANZ 6,7, and Melampsora lini, race 3, on chemically defined Cited by: A pure culture is a container with medium that allows the growth of a single known species or the type of microorganism.

Laboratory study mainly uses pure cultures because it allows the examination of one microorganism. Pure cultures otherwise termed as axenic cultures which means free of other living things or microbes."Media and Culture" offers a comprehensive and contemporary overview of the history, structure, and impact of media in American society.

The text stresses critical thinking, explores the links between media and our conceptions of democracy, and provides resources for more in-depth research/action/5.