Genetic and functional characterization of vWF and RMO1 involvement in osteosarcoma.

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Osteosarcomas are aggressive tumours with a 5-year survival rate of 60% for patients without overt metastases at diagnosis. A better understanding of osteosarcoma pathogenesis at the molecular level may result in improved treatment. I examined gene expression in osteosarcoma and observed that two genes were differentially expressed in 25 primary and metastatic tumour samples: the novel gene RMO1 (r&barbelow;educed expression in m&barbelow;etastatic o&barbelow;steosarcoma) and the von Willebrand factor (vWF) gene.vWF, previously considered to be expressed exclusively by endothelial cells and megakaryocytes, is involved in processes critical to hematogenous tumour cell metastasis to the lung. I found that vWF gene expression increased during tumour progression from primary tumour to metastasis and, unexpectedly, that vWF protein was expressed by osteosarcoma tumour cells in vivo . These findings suggest that vWF expression is deregulated in osteosarcoma, potentially contributing to metastasis.I observed that RMO1 expression decreased as tumours progressed to metastatases and, furthermore, that RMO1 exhibited frequent loss of heterozygosity in primary osteosarcoma samples, consistent with a role in cancer. RMO1 contains an open reading frame with multiple splice forms and encodes a protein with homology with the Grb7 family of adapter proteins. I determined that RMO1 is temporarily phosphorylated in response to serum treatment. Furthermore, I established that RMO1 interacts with vinculin, a cancer-related protein that regulates lamellipodia protrusion, and that the two proteins colocalize to the cellular leading edge. These results suggest RMO1 may act as an adapter protein and link cell signalling with regulation of lamellipodia, possibly through the novel interaction with vinculin. Taken together, these data are consistent with a possible role for RMO1 in inhibiting osteosarcoma progression.The comparison of gene expression in osteosarcoma tumour samples, and the further investigation of differentially expressed genes, has improved our understanding of osteosarcoma etiology. vWF is deregulated in osteosarcoma cells, suggesting a possible mechanism for osteosarcoma metastasis to the lung. Furthermore, the novel gene RMO1, observed to be frequently deleted and with altered expression in osteosarcoma metastases and a possible link between cell signalling and control of lamellipodia, may be a candidate for a protein involved in osteosarcoma development.

The Physical Object
Pagination175 leaves.
ID Numbers
Open LibraryOL21302912M
ISBN 100494077433

Conventional Osteosarcoma. Complex and largely inconsistent genetic alterations are typical of conventional osteosarcoma. Overall, some frequent genetic Genetic and functional characterization of vWF and RMO1 involvement in osteosarcoma.

book in conventional osteosarcoma are losses of portions of chromosomes 3q, 6q, 9, 10, 13, 17p, and 18q and gains of portions of chromosomes 1p, 1q, 6p, 8q, and 17p (Table 1; Figure 1).In Cited by:   Osteosarcoma is the most common type of bone average age at diagnosis is Boys and girls have a similar incidence of this tumor until late adolescence, at which time boys are more commonly affected.

In rare cases, osteosarcoma occurs in adults. Although osteosarcoma tends to occur in the larger bones, such as the shin (near the knee).

PATHOGENESIS, BIOLOGY, AND CONVENTIONAL THERAPY. Genetic aberrations or changes in gene expression in osteosarcoma tissues have not identified common or recurrent genetic lesions or pathway alterations that explain the development of this tumor type.

4,5 Rather, osteosarcoma is best characterized by its disorganized genome. Indeed, the most consistent Cited by: Osteosarcoma is a primary bone malignancy with a particularly high incidence rate in children and adolescents relative to other age groups.

The etiology of this often aggressive cancer is currently unknown, because complicated structural and numeric genomic rearrangements in cancer cells preclude understanding of tumour development.

In addition, few consistent genetic changes Cited by: The study, finding that the genetic susceptibility to young onset osteosarcoma is distinct from older adult onset osteosarcoma, was published online Apin the Journal of the National Cancer Institute. Lisa Mirabello, Ph.D., of the National Cancer Institute’s (NCI) Division of Cancer Epidemiology and Genetics, led the research.

Many people know dogs that have died from osteosarcoma (bone cancer). Some breeds seem to be predisposed to this cancers. Some breeds seem to be predisposed to this cancers. The AKC Canine Health Foundation is funding a study (led by Drs.

Kerstin Lindblad-Toh and Kenine Comstock) to determine the genetic basis of these cancers. Genetic Studies of Osteosarcoma Investigators are conducting genetic studies of osteosarcoma to better understand the role that genetic variation plays in risk and patient outcomes, and to identify new genes or genomic regions that may be important in osteosarcoma pathogenesis.

However, to translate these findings into a deeper understanding of cancer biology, analyzing the functional impact of cancer-associated genetic aberration is essential. Here I investigate how to accelerate the functional characterization of two classes of genetic alterations, point mutations and : Eejung Kim.

The Genetics of Osteosarcoma Jeff W. Mart in, 1 Jeremy A. Squire, 2 and Maria Zielenska 1 1 Department of Paediatric Laboratory Medicine, Hospital for Sick Children, T. The TARGET Osteosarcoma (OS) project elucidates comprehensive molecular characterization to determine the genetic changes that drive the initiation and progression of high-risk or hard-to-treat childhood OS project has produced comprehensive genomic profiles of nearly clinically annotated patient cases within the discovery dataset.

The treatment of sarcomas has been challenging due to their heterogeneity, rarity in the general population, relative insensitivity to chemotherapeutics, and lack of effective targeted agents. One of the first major breakthroughs in the treatment of sarcomas was the use of imatinib to treat gastrointestinal stromal tumors (GISTs).

Since then, advanced molecular techniques Cited by: 5. Bone cancer in dogs is affected by a variety of genetic risk factors. Researchers from Uppsala University, Sweden, and the Broad Institute show this in. Characterization of WWOX Tumor Suppressor Gene in Osteosarcoma.

By Jilong Yang, MD, PhD and Wei Zhang, PhD. Introduction.

Description Genetic and functional characterization of vWF and RMO1 involvement in osteosarcoma. EPUB

Osteosarcoma is the most common primary malignancy of bone in children and adolescents. It is an aggressive neoplasm composed of spindle cells producing osteoid, and can occur in any bone, but most frequently occurs in the long bones of.

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We will be providing unlimited waivers of publication charges for accepted articles related to COVID 1. Introduction. Cancer of the bones and joints is a rare genetic disease. In the United States, about new diagnoses and approximately deaths as a result of the disease are projected for The three most common forms of primary bone cancer are osteosarcoma, Ewing tumors, and by:   Osteosarcomas (OSs) are functionally defined tumors, and the function may be affected by two endogenous factors: cell-of-origin and genetic alterations.

Since the identification of mutations of two tumor suppressor genes, RB1 and p53, a large number of studies have been conducted to identify genetic alterations of : Junya Toguchida. Cellular and Phenotypic Characterization of Canine Osteosarcoma Cell Lines Marie E. Legare, Jamie Bush, Amanda K.

Ashley, Taka Kato, William H. Hanneman The Center for Environmental Medicine, Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort.

Osteosarcoma is a form of bone cancer that is most common during teenage growth spurts, when bones are developing rapidly. It usually forms in the osteoblast cells that make up the outer layer of. Osteosarcoma is a malignant bone tumor, often associated with copy number alterations, that most commonly arises in the metaphyseal ends of long bones [1–3].The survival of patients with osteosarcoma has not improved significantly in recent years and the prognosis of patients with metastatic osteosarcoma is especially poor [].Identification of prognosis markers Cited by: Chromosomal instability (CIN) is the result of increased or decreased chromosome number in a cell.

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Boveri postulated more than years ago that CIN contributes to cancer initiation and/or progression. A large amount of data collected from human tumors such as osteosarcoma suggests that CIN has a causative role in tumorigenesis. Osteosarcoma (OS) is a bone malignancy which occurs primarily in adolescents.

Since it occurs during a period of rapid growth, genes important in bone formation and growth are plausible modifiers of risk. Genes involved in DNA repair and ribosomal function may contribute to OS pathogenesis, because they maintain the integrity of critical cellular by: A larger study which pro led cases of canine osteosarcoma by 1 Mb aCGH also demonstrated a high occurrence of genetic imbalances similar to human osteosarcoma and identi ed several new.

Genetic variability of genes involved in DNA repair influence treatment outcome in osteosarcoma M.J. Wang, Y. Zhu, X.J. Guo and Z.Z. Tian Department of Orthopaedics, Xinxiang Central Hospital, Xinxiang, China Corresponding author: M.J.

Wang E-mail: [email protected] Genet. Mol. Res. 14 (3): () Received March 3, Cited by: 4. pathway in osteosarcoma to find gene alterations suchas point mutations, which can give us a clue and an understanding of the development and process of the osteosarcoma.

Methods: DNA and RNA was isolated from 15 osteosarcoma tumor samples, 3 osteosarcoma cell lines (SAOS-2, HOS, MG63) and 20 control persons.

Osteosarcoma is the most common malignant bone tumor in children and adolescents, with about new cases in the United States each year. Osteosarcoma has a high rate of metastasis, and the vast.

Using a multi-stage genetic screen working with Dr. David Schwartz, Dr.

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Greg Cosgrove, and Dr. Tasha Fingerlin we have identified a genetic risk factor for IPF carried by 67% of IPF patients. This genetic variant is in the promoter region of the MUC5B gene and based on preliminary analyses is expected to alter expression of the MUC5B gene.

Epidemiology: The age of presentation of osteosarcoma occurs chiefly in two groups: 10 ­ 25 years of age, and more than 60 years of age.

Osteosarcoma may arise as a de novo lesion or develop secondarily to a known premalignant lesion such as Paget disease, osteogenesis imperfecta, bone lesion, chronic osteomyelitis, fibrous dysplasia, giant cell tumor. Branden Moriarity, PhD, uses mouse models to identify cancer genes that cause osteosarcoma, a rare pediatric bone cancer, that primarily affects young adults.

Additionally, osteosarcoma is a highly prevalent cancer in canines, with more t cases per year in the U.S. Unlike some tumors, osteosarcoma is genetically complicated and difficult to. Phenotypic and functional analysis of lymphocytes in ltrating osteolytic tumors: use as a possible therapeutic approach of osteosarcoma.

Sandrine Th eoleyre, Kanji Mori, Bertrand Cherrier, Norbert Passuti, Fran˘cois phenotypic and functional characterization of TIL isolated from adult bone-associated tumors.

Study of Genetic Variants as Osteosarcoma Risk Factors (COG) DCEG investigators are currently collaborating with U.S. Children's Oncology Group (COG) investigators from the University of Minnesota, in the implementation and conduct of specimen collection from newly-diagnosed osteosarcoma patients now enrolling on various COG protocols.

Osteosarcoma It is a bimodal age distribution with the largest peak at an early age (children & young adults) and a second smaller peak in the elderly. Youth: Metaphysis of long bones.Introduction. Osteosarcoma is the most common malignant bone tumor in children and adolescents, with approximately new cases each year in the United States (Ottaviani and Jaffe, ).Although most cases are sporadic, the risk of osteosarcoma is increased in patients with various genetic diseases, including hereditary retinoblastoma, Li Fraumeni syndrome, and Cited by: Conclusions: Osteosarcoma contains population of cancer stem-like cells with attributes of mesenchymal stem cells, able to self-renew and to generate differentiated progeny phenotypically similar to their parental cells.

Keywords: osteosarcoma, cancer stem cells, differentiation, [18F]FDG, doxorubicinAuthor: Sara Cristina Inocêncio Neves.